rat igg 2a 2a3 antibody Search Results


anti cd25 pe  (Miltenyi Biotec)
96
Miltenyi Biotec anti cd25 pe
Anti Cd25 Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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anti cd25  (fluidigm)
95
fluidigm anti cd25

Anti Cd25, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat igg2a 2a3 antibody  (Bio X Cell)
90
Bio X Cell rat igg2a 2a3 antibody

Rat Igg2a 2a3 Antibody, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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rat igg2a isotype control  (Bio X Cell)
98
Bio X Cell rat igg2a isotype control
Neutrophils Kill Endothelial Cells (A) Endothelial cell microscopic features of chimeric mice described in <xref ref-type=Figure 6 D expressed as the percentage of mononuclear cells (mono) or PMN-containing fields that present with the indicated lesions. (B) Representative FACS dot plots of peripheral blood cells of mice treated 8 hr earlier with Ly6G-depleting Ab (1A8) or isotype control (2A3). The arrow in the FSC/SSC panel indicates granulocytic cells, the percentage of Lin − CD115 − granulocytes are indicated in red. n = 3 mice per group, mean ± SEM. (C) Presence of intravascular mononuclear (left) and polymorphonuclear cells (right) as quantified by TEM in mice treated with 1A8 or 2A3 8 hr before kidney painting with R848. n = 3 mice per group, mean ± SEM. (D) Endothelial cell microscopic features of granulocyte-depleted and control mice. n = 3 mice per group, mean ± SEM. (E) Representative peritubular capillary containing a monocyte from a 1A8-treated mouse. (F) Proinflammatory cytokine production in vitro by sorted Ly6C low and Ly6C + monocytes after 24 hr stimulation with medium alone or R848 (top) in the absence or presence of a MEK inhibitor (PD) or for medium alone or LPS (bottom) in the absence or presence of the MEK inhibitor (PD) (bottom). Multiplexed ELISA, n = 3 mice per condition. (G) Schematic representation of the molecular and cellular features of the interaction of Ly6C low monocytes with the endothelium in a steady state and TLR7-mediated endothelial “safe disposal.” Also see Figure S3 . " width="250" height="auto" />
Rat Igg2a Isotype Control, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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isotypic control  (Bio X Cell)
98
Bio X Cell isotypic control
Neutrophils Kill Endothelial Cells (A) Endothelial cell microscopic features of chimeric mice described in <xref ref-type=Figure 6 D expressed as the percentage of mononuclear cells (mono) or PMN-containing fields that present with the indicated lesions. (B) Representative FACS dot plots of peripheral blood cells of mice treated 8 hr earlier with Ly6G-depleting Ab (1A8) or isotype control (2A3). The arrow in the FSC/SSC panel indicates granulocytic cells, the percentage of Lin − CD115 − granulocytes are indicated in red. n = 3 mice per group, mean ± SEM. (C) Presence of intravascular mononuclear (left) and polymorphonuclear cells (right) as quantified by TEM in mice treated with 1A8 or 2A3 8 hr before kidney painting with R848. n = 3 mice per group, mean ± SEM. (D) Endothelial cell microscopic features of granulocyte-depleted and control mice. n = 3 mice per group, mean ± SEM. (E) Representative peritubular capillary containing a monocyte from a 1A8-treated mouse. (F) Proinflammatory cytokine production in vitro by sorted Ly6C low and Ly6C + monocytes after 24 hr stimulation with medium alone or R848 (top) in the absence or presence of a MEK inhibitor (PD) or for medium alone or LPS (bottom) in the absence or presence of the MEK inhibitor (PD) (bottom). Multiplexed ELISA, n = 3 mice per condition. (G) Schematic representation of the molecular and cellular features of the interaction of Ly6C low monocytes with the endothelium in a steady state and TLR7-mediated endothelial “safe disposal.” Also see Figure S3 . " width="250" height="auto" />
Isotypic Control, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 1 article reviews
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rat igg2a  (Bio X Cell)
97
Bio X Cell rat igg2a
Neutrophils Kill Endothelial Cells (A) Endothelial cell microscopic features of chimeric mice described in <xref ref-type=Figure 6 D expressed as the percentage of mononuclear cells (mono) or PMN-containing fields that present with the indicated lesions. (B) Representative FACS dot plots of peripheral blood cells of mice treated 8 hr earlier with Ly6G-depleting Ab (1A8) or isotype control (2A3). The arrow in the FSC/SSC panel indicates granulocytic cells, the percentage of Lin − CD115 − granulocytes are indicated in red. n = 3 mice per group, mean ± SEM. (C) Presence of intravascular mononuclear (left) and polymorphonuclear cells (right) as quantified by TEM in mice treated with 1A8 or 2A3 8 hr before kidney painting with R848. n = 3 mice per group, mean ± SEM. (D) Endothelial cell microscopic features of granulocyte-depleted and control mice. n = 3 mice per group, mean ± SEM. (E) Representative peritubular capillary containing a monocyte from a 1A8-treated mouse. (F) Proinflammatory cytokine production in vitro by sorted Ly6C low and Ly6C + monocytes after 24 hr stimulation with medium alone or R848 (top) in the absence or presence of a MEK inhibitor (PD) or for medium alone or LPS (bottom) in the absence or presence of the MEK inhibitor (PD) (bottom). Multiplexed ELISA, n = 3 mice per condition. (G) Schematic representation of the molecular and cellular features of the interaction of Ly6C low monocytes with the endothelium in a steady state and TLR7-mediated endothelial “safe disposal.” Also see Figure S3 . " width="250" height="auto" />
Rat Igg2a, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat igg2a - by Bioz Stars, 2026-02
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invivoplus rat igg2a  (Bio X Cell)
96
Bio X Cell invivoplus rat igg2a
Neutrophils Kill Endothelial Cells (A) Endothelial cell microscopic features of chimeric mice described in <xref ref-type=Figure 6 D expressed as the percentage of mononuclear cells (mono) or PMN-containing fields that present with the indicated lesions. (B) Representative FACS dot plots of peripheral blood cells of mice treated 8 hr earlier with Ly6G-depleting Ab (1A8) or isotype control (2A3). The arrow in the FSC/SSC panel indicates granulocytic cells, the percentage of Lin − CD115 − granulocytes are indicated in red. n = 3 mice per group, mean ± SEM. (C) Presence of intravascular mononuclear (left) and polymorphonuclear cells (right) as quantified by TEM in mice treated with 1A8 or 2A3 8 hr before kidney painting with R848. n = 3 mice per group, mean ± SEM. (D) Endothelial cell microscopic features of granulocyte-depleted and control mice. n = 3 mice per group, mean ± SEM. (E) Representative peritubular capillary containing a monocyte from a 1A8-treated mouse. (F) Proinflammatory cytokine production in vitro by sorted Ly6C low and Ly6C + monocytes after 24 hr stimulation with medium alone or R848 (top) in the absence or presence of a MEK inhibitor (PD) or for medium alone or LPS (bottom) in the absence or presence of the MEK inhibitor (PD) (bottom). Multiplexed ELISA, n = 3 mice per condition. (G) Schematic representation of the molecular and cellular features of the interaction of Ly6C low monocytes with the endothelium in a steady state and TLR7-mediated endothelial “safe disposal.” Also see Figure S3 . " width="250" height="auto" />
Invivoplus Rat Igg2a, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/invivoplus rat igg2a/product/Bio X Cell
Average 96 stars, based on 1 article reviews
invivoplus rat igg2a - by Bioz Stars, 2026-02
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control rat igg  (Bio X Cell)
95
Bio X Cell control rat igg
Neutrophils Kill Endothelial Cells (A) Endothelial cell microscopic features of chimeric mice described in <xref ref-type=Figure 6 D expressed as the percentage of mononuclear cells (mono) or PMN-containing fields that present with the indicated lesions. (B) Representative FACS dot plots of peripheral blood cells of mice treated 8 hr earlier with Ly6G-depleting Ab (1A8) or isotype control (2A3). The arrow in the FSC/SSC panel indicates granulocytic cells, the percentage of Lin − CD115 − granulocytes are indicated in red. n = 3 mice per group, mean ± SEM. (C) Presence of intravascular mononuclear (left) and polymorphonuclear cells (right) as quantified by TEM in mice treated with 1A8 or 2A3 8 hr before kidney painting with R848. n = 3 mice per group, mean ± SEM. (D) Endothelial cell microscopic features of granulocyte-depleted and control mice. n = 3 mice per group, mean ± SEM. (E) Representative peritubular capillary containing a monocyte from a 1A8-treated mouse. (F) Proinflammatory cytokine production in vitro by sorted Ly6C low and Ly6C + monocytes after 24 hr stimulation with medium alone or R848 (top) in the absence or presence of a MEK inhibitor (PD) or for medium alone or LPS (bottom) in the absence or presence of the MEK inhibitor (PD) (bottom). Multiplexed ELISA, n = 3 mice per condition. (G) Schematic representation of the molecular and cellular features of the interaction of Ly6C low monocytes with the endothelium in a steady state and TLR7-mediated endothelial “safe disposal.” Also see Figure S3 . " width="250" height="auto" />
Control Rat Igg, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
control rat igg - by Bioz Stars, 2026-02
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anti-cd25 [2a3]  (Becton Dickinson)
90
Becton Dickinson anti-cd25 [2a3]
Neutrophils Kill Endothelial Cells (A) Endothelial cell microscopic features of chimeric mice described in <xref ref-type=Figure 6 D expressed as the percentage of mononuclear cells (mono) or PMN-containing fields that present with the indicated lesions. (B) Representative FACS dot plots of peripheral blood cells of mice treated 8 hr earlier with Ly6G-depleting Ab (1A8) or isotype control (2A3). The arrow in the FSC/SSC panel indicates granulocytic cells, the percentage of Lin − CD115 − granulocytes are indicated in red. n = 3 mice per group, mean ± SEM. (C) Presence of intravascular mononuclear (left) and polymorphonuclear cells (right) as quantified by TEM in mice treated with 1A8 or 2A3 8 hr before kidney painting with R848. n = 3 mice per group, mean ± SEM. (D) Endothelial cell microscopic features of granulocyte-depleted and control mice. n = 3 mice per group, mean ± SEM. (E) Representative peritubular capillary containing a monocyte from a 1A8-treated mouse. (F) Proinflammatory cytokine production in vitro by sorted Ly6C low and Ly6C + monocytes after 24 hr stimulation with medium alone or R848 (top) in the absence or presence of a MEK inhibitor (PD) or for medium alone or LPS (bottom) in the absence or presence of the MEK inhibitor (PD) (bottom). Multiplexed ELISA, n = 3 mice per condition. (G) Schematic representation of the molecular and cellular features of the interaction of Ly6C low monocytes with the endothelium in a steady state and TLR7-mediated endothelial “safe disposal.” Also see Figure S3 . " width="250" height="auto" />
Anti Cd25 [2a3], supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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rat igg2a 2a3  (Bio X Cell)
96
Bio X Cell rat igg2a 2a3
Neutrophils Kill Endothelial Cells (A) Endothelial cell microscopic features of chimeric mice described in <xref ref-type=Figure 6 D expressed as the percentage of mononuclear cells (mono) or PMN-containing fields that present with the indicated lesions. (B) Representative FACS dot plots of peripheral blood cells of mice treated 8 hr earlier with Ly6G-depleting Ab (1A8) or isotype control (2A3). The arrow in the FSC/SSC panel indicates granulocytic cells, the percentage of Lin − CD115 − granulocytes are indicated in red. n = 3 mice per group, mean ± SEM. (C) Presence of intravascular mononuclear (left) and polymorphonuclear cells (right) as quantified by TEM in mice treated with 1A8 or 2A3 8 hr before kidney painting with R848. n = 3 mice per group, mean ± SEM. (D) Endothelial cell microscopic features of granulocyte-depleted and control mice. n = 3 mice per group, mean ± SEM. (E) Representative peritubular capillary containing a monocyte from a 1A8-treated mouse. (F) Proinflammatory cytokine production in vitro by sorted Ly6C low and Ly6C + monocytes after 24 hr stimulation with medium alone or R848 (top) in the absence or presence of a MEK inhibitor (PD) or for medium alone or LPS (bottom) in the absence or presence of the MEK inhibitor (PD) (bottom). Multiplexed ELISA, n = 3 mice per condition. (G) Schematic representation of the molecular and cellular features of the interaction of Ly6C low monocytes with the endothelium in a steady state and TLR7-mediated endothelial “safe disposal.” Also see Figure S3 . " width="250" height="auto" />
Rat Igg2a 2a3, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat igg2a 2a3 - by Bioz Stars, 2026-02
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anti-cd25 pecy7  (Becton Dickinson)
90
Becton Dickinson anti-cd25 pecy7
Multivariable regression analysis of the effects of age and baseline immune parameters on the magnitude of peak VZV-specific Th1 immune responses to ZVL in the primary cohort ( n = 399) a
Anti Cd25 Pecy7, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bgviolet450 rat igg2a  (Biogems International)
90
Biogems International bgviolet450 rat igg2a
Figure 5. TANs drive B-cell differentiation independently of T cells. A, Quantification of CD138 expression on splenic B220þ cells following various culture conditions—when cultured alone (B), with T cells (B þ T, ratio 1:1), with TANs (B þ TAN, ratio 1:1), with TAN and T cells (B þ TAN þ T, ratio 1:1:1), with T cells but no contact allowed with TANs (B þT//TAN,ratio 1:1:1), or TANs cocultured with T cells but no contact allowed with B cells (B//T þTAN, ratio 1:1:1). Data represent the mean SEM (n ¼ 6–9; , P < 0.001; n.s., not significant). Groups were compared using one-way ANOVA. B, Representative flow plots displaying CD138 expression out of total B220þ population in the different coculture conditions. C, Quantification of <t>IgG</t> release to the media following overnight coculture of isolated splenic B220þ
Bgviolet450 Rat Igg2a, supplied by Biogems International, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Journal: iScience

Article Title: Defects in NK cell immunity of pediatric cancer patients revealed by deep immune profiling

doi: 10.1016/j.isci.2024.110837

Figure Lengend Snippet:

Article Snippet: Anti-CD25 (clone 2A3, conjugated to 149Sm) , Standard BioTools , Cat#3149010B.

Techniques: Purification, Clinical Proteomics, Recombinant, Blocking Assay, Staining, Saline, Mass Cytometry, Software, Cytometry

Neutrophils Kill Endothelial Cells (A) Endothelial cell microscopic features of chimeric mice described in <xref ref-type=Figure 6 D expressed as the percentage of mononuclear cells (mono) or PMN-containing fields that present with the indicated lesions. (B) Representative FACS dot plots of peripheral blood cells of mice treated 8 hr earlier with Ly6G-depleting Ab (1A8) or isotype control (2A3). The arrow in the FSC/SSC panel indicates granulocytic cells, the percentage of Lin − CD115 − granulocytes are indicated in red. n = 3 mice per group, mean ± SEM. (C) Presence of intravascular mononuclear (left) and polymorphonuclear cells (right) as quantified by TEM in mice treated with 1A8 or 2A3 8 hr before kidney painting with R848. n = 3 mice per group, mean ± SEM. (D) Endothelial cell microscopic features of granulocyte-depleted and control mice. n = 3 mice per group, mean ± SEM. (E) Representative peritubular capillary containing a monocyte from a 1A8-treated mouse. (F) Proinflammatory cytokine production in vitro by sorted Ly6C low and Ly6C + monocytes after 24 hr stimulation with medium alone or R848 (top) in the absence or presence of a MEK inhibitor (PD) or for medium alone or LPS (bottom) in the absence or presence of the MEK inhibitor (PD) (bottom). Multiplexed ELISA, n = 3 mice per condition. (G) Schematic representation of the molecular and cellular features of the interaction of Ly6C low monocytes with the endothelium in a steady state and TLR7-mediated endothelial “safe disposal.” Also see Figure S3 . " width="100%" height="100%">

Journal: Cell

Article Title: Nr4a1 -Dependent Ly6C low Monocytes Monitor Endothelial Cells and Orchestrate Their Disposal

doi: 10.1016/j.cell.2013.03.010

Figure Lengend Snippet: Neutrophils Kill Endothelial Cells (A) Endothelial cell microscopic features of chimeric mice described in Figure 6 D expressed as the percentage of mononuclear cells (mono) or PMN-containing fields that present with the indicated lesions. (B) Representative FACS dot plots of peripheral blood cells of mice treated 8 hr earlier with Ly6G-depleting Ab (1A8) or isotype control (2A3). The arrow in the FSC/SSC panel indicates granulocytic cells, the percentage of Lin − CD115 − granulocytes are indicated in red. n = 3 mice per group, mean ± SEM. (C) Presence of intravascular mononuclear (left) and polymorphonuclear cells (right) as quantified by TEM in mice treated with 1A8 or 2A3 8 hr before kidney painting with R848. n = 3 mice per group, mean ± SEM. (D) Endothelial cell microscopic features of granulocyte-depleted and control mice. n = 3 mice per group, mean ± SEM. (E) Representative peritubular capillary containing a monocyte from a 1A8-treated mouse. (F) Proinflammatory cytokine production in vitro by sorted Ly6C low and Ly6C + monocytes after 24 hr stimulation with medium alone or R848 (top) in the absence or presence of a MEK inhibitor (PD) or for medium alone or LPS (bottom) in the absence or presence of the MEK inhibitor (PD) (bottom). Multiplexed ELISA, n = 3 mice per condition. (G) Schematic representation of the molecular and cellular features of the interaction of Ly6C low monocytes with the endothelium in a steady state and TLR7-mediated endothelial “safe disposal.” Also see Figure S3 .

Article Snippet: Rat IgG2a isotype control (2A3; Bio X Cell or BD PharMingen) anti-mouse Ly6G PE (1A8; BD Pharmingen), Anti-mouse CD115 FITC (CSF1R; AFS98 BD PharMingen), Anti-mouse CD16/32 (2.4G2BD Pharmingen), Anti-mouse CD3 Biotin (145 2C-11; BD PharMingen), Anti-mouse NK1.1Biotin (PK136; BD Pharmingen), Anti-mouse CD19 Biotin (1D3; BD PharMingen).

Techniques: In Vitro, Enzyme-linked Immunosorbent Assay

Multivariable regression analysis of the effects of age and baseline immune parameters on the magnitude of peak VZV-specific Th1 immune responses to ZVL in the primary cohort ( n = 399) a

Journal: Journal of Virology

Article Title: The Effect of Age on the Immunogenicity of the Live Attenuated Zoster Vaccine Is Predicted by Baseline Regulatory T Cells and Varicella-Zoster Virus-Specific T Cell Immunity

doi: 10.1128/JVI.00305-19

Figure Lengend Snippet: Multivariable regression analysis of the effects of age and baseline immune parameters on the magnitude of peak VZV-specific Th1 immune responses to ZVL in the primary cohort ( n = 399) a

Article Snippet: Cells were stained with viability dye (catalog number 423104; BioLegend) and then surface stained with anti-ICOS Ax488 (Alexa Fluor 488) (clone C398.4A, catalog number 313514; BioLegend), anti-CTLA4 PE-CF594 (cyanine-based fluorescent 594) (clone BNI3, catalog number 562742; BD), anti-LAG3 PerCP-Cy5.5 (clone 11C3C65, catalog number 369312; BioLegend), anti-CD25 PECy7 (clone 2A3, catalog number 335807; BD), anti-CD39 APC (clone TU66, catalog number 560239; BD), anti-CD4 Ax700 (clone RPA-T4, catalog number 557922; BD), anti-CD3 APC-H7 (clone SK7, catalog number 560176; BD), and anti-CD127 BV421 (clone A019D5, catalog number 351310; BioLegend).

Techniques:

Multivariable regression analysis of the effects of age and baseline immune parameters on the magnitude of peak VZV-specific CTL and Tfh responses to ZVL in the VZV-specific flow cytometry subset of the primary cohort ( n = 95) a

Journal: Journal of Virology

Article Title: The Effect of Age on the Immunogenicity of the Live Attenuated Zoster Vaccine Is Predicted by Baseline Regulatory T Cells and Varicella-Zoster Virus-Specific T Cell Immunity

doi: 10.1128/JVI.00305-19

Figure Lengend Snippet: Multivariable regression analysis of the effects of age and baseline immune parameters on the magnitude of peak VZV-specific CTL and Tfh responses to ZVL in the VZV-specific flow cytometry subset of the primary cohort ( n = 95) a

Article Snippet: Cells were stained with viability dye (catalog number 423104; BioLegend) and then surface stained with anti-ICOS Ax488 (Alexa Fluor 488) (clone C398.4A, catalog number 313514; BioLegend), anti-CTLA4 PE-CF594 (cyanine-based fluorescent 594) (clone BNI3, catalog number 562742; BD), anti-LAG3 PerCP-Cy5.5 (clone 11C3C65, catalog number 369312; BioLegend), anti-CD25 PECy7 (clone 2A3, catalog number 335807; BD), anti-CD39 APC (clone TU66, catalog number 560239; BD), anti-CD4 Ax700 (clone RPA-T4, catalog number 557922; BD), anti-CD3 APC-H7 (clone SK7, catalog number 560176; BD), and anti-CD127 BV421 (clone A019D5, catalog number 351310; BioLegend).

Techniques: Flow Cytometry

Multivariable regression analysis of the effects of age and baseline immune parameters on the magnitude of persistent VZV-specific Th1 cells 1 year after ZVL administration in the primary cohort ( n = 399) a

Journal: Journal of Virology

Article Title: The Effect of Age on the Immunogenicity of the Live Attenuated Zoster Vaccine Is Predicted by Baseline Regulatory T Cells and Varicella-Zoster Virus-Specific T Cell Immunity

doi: 10.1128/JVI.00305-19

Figure Lengend Snippet: Multivariable regression analysis of the effects of age and baseline immune parameters on the magnitude of persistent VZV-specific Th1 cells 1 year after ZVL administration in the primary cohort ( n = 399) a

Article Snippet: Cells were stained with viability dye (catalog number 423104; BioLegend) and then surface stained with anti-ICOS Ax488 (Alexa Fluor 488) (clone C398.4A, catalog number 313514; BioLegend), anti-CTLA4 PE-CF594 (cyanine-based fluorescent 594) (clone BNI3, catalog number 562742; BD), anti-LAG3 PerCP-Cy5.5 (clone 11C3C65, catalog number 369312; BioLegend), anti-CD25 PECy7 (clone 2A3, catalog number 335807; BD), anti-CD39 APC (clone TU66, catalog number 560239; BD), anti-CD4 Ax700 (clone RPA-T4, catalog number 557922; BD), anti-CD3 APC-H7 (clone SK7, catalog number 560176; BD), and anti-CD127 BV421 (clone A019D5, catalog number 351310; BioLegend).

Techniques:

Multivariable regression analysis of the effects of age and baseline and peak immune parameters on the magnitude of persistent VZV-specific Th1 cells 1 year after ZVL administration in the primary cohort ( n = 399) a

Journal: Journal of Virology

Article Title: The Effect of Age on the Immunogenicity of the Live Attenuated Zoster Vaccine Is Predicted by Baseline Regulatory T Cells and Varicella-Zoster Virus-Specific T Cell Immunity

doi: 10.1128/JVI.00305-19

Figure Lengend Snippet: Multivariable regression analysis of the effects of age and baseline and peak immune parameters on the magnitude of persistent VZV-specific Th1 cells 1 year after ZVL administration in the primary cohort ( n = 399) a

Article Snippet: Cells were stained with viability dye (catalog number 423104; BioLegend) and then surface stained with anti-ICOS Ax488 (Alexa Fluor 488) (clone C398.4A, catalog number 313514; BioLegend), anti-CTLA4 PE-CF594 (cyanine-based fluorescent 594) (clone BNI3, catalog number 562742; BD), anti-LAG3 PerCP-Cy5.5 (clone 11C3C65, catalog number 369312; BioLegend), anti-CD25 PECy7 (clone 2A3, catalog number 335807; BD), anti-CD39 APC (clone TU66, catalog number 560239; BD), anti-CD4 Ax700 (clone RPA-T4, catalog number 557922; BD), anti-CD3 APC-H7 (clone SK7, catalog number 560176; BD), and anti-CD127 BV421 (clone A019D5, catalog number 351310; BioLegend).

Techniques:

Expression of FOXP3 and inhibitory ligands on CD4+ CD25+ CD127− Treg. Data were derived from 6 adults of all ages. The parent population is indicated on the abscissa. Horizontal bars indicate paired comparisons and P values.

Journal: Journal of Virology

Article Title: The Effect of Age on the Immunogenicity of the Live Attenuated Zoster Vaccine Is Predicted by Baseline Regulatory T Cells and Varicella-Zoster Virus-Specific T Cell Immunity

doi: 10.1128/JVI.00305-19

Figure Lengend Snippet: Expression of FOXP3 and inhibitory ligands on CD4+ CD25+ CD127− Treg. Data were derived from 6 adults of all ages. The parent population is indicated on the abscissa. Horizontal bars indicate paired comparisons and P values.

Article Snippet: Cells were stained with viability dye (catalog number 423104; BioLegend) and then surface stained with anti-ICOS Ax488 (Alexa Fluor 488) (clone C398.4A, catalog number 313514; BioLegend), anti-CTLA4 PE-CF594 (cyanine-based fluorescent 594) (clone BNI3, catalog number 562742; BD), anti-LAG3 PerCP-Cy5.5 (clone 11C3C65, catalog number 369312; BioLegend), anti-CD25 PECy7 (clone 2A3, catalog number 335807; BD), anti-CD39 APC (clone TU66, catalog number 560239; BD), anti-CD4 Ax700 (clone RPA-T4, catalog number 557922; BD), anti-CD3 APC-H7 (clone SK7, catalog number 560176; BD), and anti-CD127 BV421 (clone A019D5, catalog number 351310; BioLegend).

Techniques: Expressing, Derivative Assay

Figure 5. TANs drive B-cell differentiation independently of T cells. A, Quantification of CD138 expression on splenic B220þ cells following various culture conditions—when cultured alone (B), with T cells (B þ T, ratio 1:1), with TANs (B þ TAN, ratio 1:1), with TAN and T cells (B þ TAN þ T, ratio 1:1:1), with T cells but no contact allowed with TANs (B þT//TAN,ratio 1:1:1), or TANs cocultured with T cells but no contact allowed with B cells (B//T þTAN, ratio 1:1:1). Data represent the mean SEM (n ¼ 6–9; , P < 0.001; n.s., not significant). Groups were compared using one-way ANOVA. B, Representative flow plots displaying CD138 expression out of total B220þ population in the different coculture conditions. C, Quantification of IgG release to the media following overnight coculture of isolated splenic B220þ

Journal: Cancer Immunology Research

Article Title: Tumor-Associated Neutrophils Drive B-cell Recruitment and Their Differentiation to Plasma Cells

doi: 10.1158/2326-6066.cir-20-0839

Figure Lengend Snippet: Figure 5. TANs drive B-cell differentiation independently of T cells. A, Quantification of CD138 expression on splenic B220þ cells following various culture conditions—when cultured alone (B), with T cells (B þ T, ratio 1:1), with TANs (B þ TAN, ratio 1:1), with TAN and T cells (B þ TAN þ T, ratio 1:1:1), with T cells but no contact allowed with TANs (B þT//TAN,ratio 1:1:1), or TANs cocultured with T cells but no contact allowed with B cells (B//T þTAN, ratio 1:1:1). Data represent the mean SEM (n ¼ 6–9; , P < 0.001; n.s., not significant). Groups were compared using one-way ANOVA. B, Representative flow plots displaying CD138 expression out of total B220þ population in the different coculture conditions. C, Quantification of IgG release to the media following overnight coculture of isolated splenic B220þ

Article Snippet: Isotype control antibodies were as follows: APCconjugated rat IgG2a (clone 2A3; Biogems), FITC-conjugated rat IgG2b (clone RTK4530; BioLegend), BGViolet450 rat IgG2a (clone 2A3, Biogems), PE-conjugated rat IgG2a (clone 2A3, Biogems).

Techniques: Cell Differentiation, Expressing, Cell Culture, Isolation

Figure 6. TANs express membranal BAFF, but not membranal APRIL, and mediate B-cell IgG production in a BAFF-R–dependent manner. A and B, Expression of membranal BAFF and APRIL in TANs within the whole tumor (A) and following TANs' isolation from the tumors (B). Representative histograms showing BAFF and APRIL expression in TANs (gated as total Ly6Gþ population) are provided (right plots). C, IgG production by splenic B cells cocultured with TANs (ratio 1:5) in the absence or presence of anti–BAFF-R antibody. Data represent the mean SEM (n ¼ 4; , P < 0.01; , P < 0.001). Groups were compared using one-way ANOVA. D, Quantification of CD138 expression on isolated splenic B220þ cells cultured alone or cocultured with TANs (ratio 1:5), without or with blocking of the three potential BAFF receptors, BAFF-R, TACI, or BCMA. Data represent the mean SEM (n ¼ 4–10; , P < 0.001; n.s., nonsignificant). Groups were compared using one-way ANOVA.

Journal: Cancer Immunology Research

Article Title: Tumor-Associated Neutrophils Drive B-cell Recruitment and Their Differentiation to Plasma Cells

doi: 10.1158/2326-6066.cir-20-0839

Figure Lengend Snippet: Figure 6. TANs express membranal BAFF, but not membranal APRIL, and mediate B-cell IgG production in a BAFF-R–dependent manner. A and B, Expression of membranal BAFF and APRIL in TANs within the whole tumor (A) and following TANs' isolation from the tumors (B). Representative histograms showing BAFF and APRIL expression in TANs (gated as total Ly6Gþ population) are provided (right plots). C, IgG production by splenic B cells cocultured with TANs (ratio 1:5) in the absence or presence of anti–BAFF-R antibody. Data represent the mean SEM (n ¼ 4; , P < 0.01; , P < 0.001). Groups were compared using one-way ANOVA. D, Quantification of CD138 expression on isolated splenic B220þ cells cultured alone or cocultured with TANs (ratio 1:5), without or with blocking of the three potential BAFF receptors, BAFF-R, TACI, or BCMA. Data represent the mean SEM (n ¼ 4–10; , P < 0.001; n.s., nonsignificant). Groups were compared using one-way ANOVA.

Article Snippet: Isotype control antibodies were as follows: APCconjugated rat IgG2a (clone 2A3; Biogems), FITC-conjugated rat IgG2b (clone RTK4530; BioLegend), BGViolet450 rat IgG2a (clone 2A3, Biogems), PE-conjugated rat IgG2a (clone 2A3, Biogems).

Techniques: Expressing, Isolation, Cell Culture, Blocking Assay